Ozono Therapy^TM is easily installed in a few minutes on the water tap, thus providing for the immediate ozone production.
When opening the tap, ozone is produced according to the out-coming water quantity. Thus Ozono Therapy^TM turns out to be versatile and suitable for professional and domestic ambiences as well, being safe and offering an immediate benefit.

INSTALLATION INSTRUCTIONS

1. The device should be positioned 10 cm above the tap height. It can be fitted and screwed to the wall, thus removing its base support.
2. Connect the silicone tube to the supply manifold on the lower portion of the device.
3. On the tap to be used, replaced the water mixer with a venturi water/ozone mixer.
4. Strands are supplied with the product, to be discretely used, to fit the ozone supply pie. Avoid any bottleneck on the pipe.
5. Connect the power supply, 220V on the right side of the ozoniser.

Ozone (O₃), discovered between late 18th and early 19th century, is an unstable substance which is derived from elemental oxygen and appears in the form of a highly reactive, bluish aerial gas with a peculiar pungent smell, identical to the one which permeates the air during a rainstorm.
Its high reactivity makes this substance unstable, thus it should be produced immediately before use. After performing its function, and thanks to this very same peculiarity, it tends to revert rapidly back into oxygen without leaving any residues. These properties give it a higher value compared to other sanitising agents used to make drinkable water.
Since it derives from oxygen, ozone can attack harmful substances found in the water and convert them into harmless products, thus making water drinkable. It can perform this function even better than oxygen itself and other similar substances, because its oxidising power is far higher, thus acting more rapidly and more efficiently.

REGULATIONS
The FDA, a federal agency of the United States Department of Health and Human Services, allows the use of ozone as an antimicrobial agent in the gaseous phase or in aqueous solution within the production processes (treatment, processing, and storage) of foods, including meat, eggs, fish, cheese, fruits, and vegetables, thus validating ozone compatibility with human activities. In particular, Document 21 CFR part 173.368 (Docket No. 00F-1482) has defined ozone as a GRAS (Generally Recognized As Safe) substance, i.e. a secondary direct food additive which is safe for human health.
The EPA (Environmental Protection Agency) recognizes the efficacy of ozone and its higher oxidising power compared to chlorine and UV radiations, while showing minor contact times and the lack of micro-organism regrowth following the ozonation treatment.
In 2003, even the European Community introduced the use of food-grade ozone for disinfection and sterilization in water bottling processes. The Italian Health Ministry issued Protocol No. 24482 on 31st July, 1996, which recognized the use of ozone in air and water treatment as an approved product for sterilization of environments contaminated by bacteria, viruses, spores, moulds and mites.

OZONE EFFICACY
Ozone is one of the strongest natural oxidants, acting in concentrations of fractions of ppm (parts per million) in a relatively short time.

Table 1: Inactivation of bacteria, viruses, moulds, fungi and insects after ozonation in water

ORGANISM	CONCENTRATION	EXPOSITION TIME
(E. Coli, Legionella, Mycobacterium, Fecal Streptococcus)	0,23 ppm – 2,2 ppm	< 20 minutes
VIRUS (Polivirus type-1, Human Rotavirus, Enteric virus)	0,2 ppm – 4,1 ppm	< 20 minutes
MOLDS (Aspergillus Niger, vari ceppi di penicillum, Cladosporium	2 ppm	60 minutes
FUNGI (Candida Parapsilosis, Candida Tropicalis	0,02 ppm – 0,26 ppm	< 1,67 minutes
INSECTS (Acarus Siro, Tyrophagus Casei, Tyrophagus Putrescientiae)	1,5 ppm – 2 ppm	30 minutes

Ozone has been recognised worldwide as a safe substance for sanitation procedures, and it stands out from among any other disinfectants because of the following properties:

• Ozone dissolved in water is safe and convenient to use;
• Since it converts back quickly into oxygen, it leaves no residues which may possibly contaminate treated articles;
• It features a high range of applications;
• It is produced on site as necessary, therefore its storage does not cause any problems;
• Its disposal does not entail any kind of costs or issues.

We would like to remark that ozone has been recognised by FDA (Food and Drug Administration) as an “adequate antimicrobial agent”, from 300 to 2000 times more effective than chlorine in killing germs. Even EPA declares the superiority of ozone in terms of efficacy as a biocide, as shown in Table 2, where Ct values (Concentration x Time) for several disinfectants used in virus inactivation are compared, demonstrating that ozone is better than chlorine in reducing the number of protozoa.

Table 2: Ct values for virus inactivation by several disinfectants

DISINFECTANT	UNIT	INACTIVATION
Chlorine	mg∙min/L	3	4	6
Chloramine	mg∙min/L	643	1.067	1.491
Chlorine dioxide	mg∙min/L	4,2	12,8	25,1
Ozone	mg∙min/L	0,5	0,8	1,0
UV	mg∙min/L	21	36	N/A

Ct values drawn from AWWA 1991 (American Water Works Association).

Table 3 shows instead the disinfection rate for Giardia spores. As it may be noticed, chlorine and chloramine have higher Ct values than ozone. This means that ozone is a strong disinfectant, surely better than other sanitising agents commonly used to inactivate this kind of micro-organism.

Table 3: Ct values for the inactivation of Giardia cysts by several disinfectants

DISINFECTANT	INACTIVATION (mg∙min/L)
	0,5-log	1-log	1,5-log	2-log	2,5-log	3-log
Chlorine2	17	35	52	69	87	104
Chloramine3	310	615	930	1.230	1.540	1.850
Chlorine dioxide4	4	7,7	12	15	19	23
Ozone	0,23	0,48	0,72	0,95	1,2	1,43

Ct values drawn from AWWA 1991 (American Water Works Association).

SANITISING FORMULA IN OZONATED WATER
Ozone is an aerial gas which is soluble in aqueous solution (up to 13 times more than oxygen at a temperature of 32 to 86°F), with an inversely proportional solubility with respect to temperature and pH. Taking advantage of this characteristic and tackling the issue of its relatively short duration (ozone tends to be decomposed quickly into oxygen without leaving any harmful residuals in the environment), we have created Ozono Therapy^TM, a water ozonator distributed exclusively by Biosteril Italia srl designed to generate a controlled ozone flow which, blended into mains water, cuts down limitations to its accessibility.

• The well-known benefits of ozonated water are first and foremost due to the oxidising and biocidal capacity of ozone itself: for this purpose the equipment is suitable for being connected to the water supply network to perform antiseptical washing.
• Secondly, the reconstitution of other active ingredients in ozonated water included in sanitising products such as Rely+On^TM Virkon^TM e Rely+On^TM Perasafe^TM (Potassium peroxymonosulfate and percarbonate and TAED combination, respectively) allows to take advantage of their oxidation synergy, greatly enhancing their efficacy as a biocide and accelerating the pathogen abatement time, thus generating strong benefits in terms of healthcare.

The acceleration of the active ingredient dynamics generated by the increased oxidation contemplates the benefits of both the former and the latter aspect by augmenting the spectrum and reducing pathogen abatement times, thus reaching the goal of a number of professionals who need to speculate on the application time of disinfectants, now more than ever.
In addition, ozonated water is immediately treated with a strong oxidant. This means that, even before reconstituting the disinfecting solution, water coming from the mains, whichever its source, is affected by a strong reduction in its bacterial load. This is a great advantage, because this way not a single active ingredient is lost by the sanitising product which, although accidentally, is focused on disinfecting the potentially contaminated mains water before sanitising the contaminated surfaces. For this reason, even when ozone is included in the solution no more, the maximum efficacy of the active ingredients contained in disinfectants is kept unaltered.

The application of the aforementioned associations can be noticed within the following kits:

• PoliDisin^TMPLUS
• PoliSteril^TMPROF
• PoliSteril^TMPLUS

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